A quantitative analysis of shear effects on cell suspension and cell culture of perilla frutescens in bioreactors

Authors

  • Jian-Jiang Zhong,

    1. International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565, Japan
    Current affiliation:
    1. Research Institute of Biochemical Engineering. East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
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  • Kazuhito Fujiyama,

    1. International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565, Japan
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  • Tatsuji Seki,

    1. International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565, Japan
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  • Toshiomi Yoshida

    1. International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565, Japan
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Abstract

The short-time effects of shear on suspended cells of Perilla frutescens were quantitatively analyzed by exposing the cells to a well-defined flow field in a rotating drum reactor. It was found that both shear rate and shearing time significantly affected cell viability. The quantitative effects of shear on cell growth and the production of anthocyanin, a secondary metabolite, by the cell cultures were further investigated in a series of batch cultivations using a 5-L plant cell bioreactor with a marine impeller. The results indicated that there was an optimum range of shear rate; i.e., an average shear rate of 20 to 30 s−1 or an impeller tip speed of 5 to 8 dm/s, which maximized all the values of the following parameters: the specific growth rate, the maximum cell concentration, the (specific) production and productivity of anthocyanin, and the cell and anthocyanin yields. © 1994 John Wiley & Sons, Inc.

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