Effect of temperature on nucleotide pools and monoclonal antibody production in a mouse hybridoma
Article first published online: 19 FEB 2004
Copyright © 1994 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 44, Issue 10, pages 1235–1245, 20 November 1994
How to Cite
Barnabé, N. and Butler, M. (1994), Effect of temperature on nucleotide pools and monoclonal antibody production in a mouse hybridoma. Biotechnol. Bioeng., 44: 1235–1245. doi: 10.1002/bit.260441011
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 15 JUL 1994
- Manuscript Received: 18 APR 1994
- monoclonal antibody;
The specific monoclonal antibody productivity (qMab) of a murine hybridoma (CC9C10) increased with incubation temperature in the range 33°C to 39°C. qMab was constant at each temperature and was independent of the phase of culture. The qMab increased 97% at 39°C and decreased by 21% at 33°C compared with controls at 37°C. Specific rates of substrate (glucose and glutamine) utilization and byproduct (lactate and ammonia) formation also increased with temperature but the yield coefficient, YLac/Llc' was constant for 33°C to 39°C and YAmm/Gin was constant for 37°C to 39°C. YAmm/Gin at 33°C was lower than the control. Changes in specific nucleotide concentrations and ratios were monitored by analysis of intracellular nucleotide pools. The NTP ratio, (ATP + GTP)/(UTP + CTP), increased and the U-ratio (UTP/UDP–GNac) decreased during the course of each culture, whereas the adenylate energy charge, (ATP + 0.5ADP)/(ATP + ADP + AMP), remained relatively constant at a value 0.8. The relative content of UDP-/N acetyl galactosamine, UDP-N acetyl glucosamine, and NAD increased with incubation temperature, whereas the relative ATP content, SA(ATP + ADP + AMP)/SU (UTP + UDP-sugars) ratio, purine/pyrimidine, ATP/GTP, and U-ratio decreased at higher incubation temperatures. It is possible that these nucleotide parameters may have a regulatory role in the changes of qMab observed at the higher temperatures. © 1994 John Wiley & Sons, Inc.