Andrew J. Griggs, Jonathan J. Stickel and James J. Lischeske
A mechanistic model for the enzymatic saccharification of cellulose has been developed. In part II, the kinetic model from part I is expanded to include solution-phase kinetics, an innovative product-inhibition scheme, and sterically hindered enzyme adsorption, based on current structural knowledge of the component enzymes. The model was used to explore several potential rate-limiting phenomena (e.g. accessibility, inhibition, degree of polymerization). Preliminary simulation results indicate that the accessibility of the substrate to the enzymes is a key rate-determining factor.