Utilization and evaluation of CHO-specific sequence databases for mass spectrometry based proteomics (pages 1386–1394)
Paula Meleady, Raimund Hoffrogge, Michael Henry, Oliver Rupp, Juan Hernandez Bort, Colin Clarke, Karina Brinkrolf, Shane Kelly, Benjamin Müller, Padraig Doolan, Matthias Hackl, Tim Frederik Beckmann, Thomas Noll, Johannes Grillari, Niall Barron, Alf Pühler, Martin Clynes and Nicole Borth
Version of Record online: 2 MAR 2012 | DOI: 10.1002/bit.24476
The availability of CHO-specific genomic and cDNA sequence information has greatly enhanced protein identification by MS based proteomics of CHO cells. Both the number of proteins that could be identified and the confidence of identification are now significantly increased. Many of the newly identified proteins are related to important cellular processes such as energy metabolism and translation, stressing the importance of these new databases in bioprocess related research.