Monoclonal antibody capture and viral clearance by cation exchange chromatography (pages 2048–2058)
G.R. Miesegaes, S. Lute, D.M. Strauss, E.K. Read, A. Venkiteshwaran, A. Kreuzman, R. Shah, P. Shamlou, D. Chen and K. Brorson
Version of Record online: 8 APR 2012 | DOI: 10.1002/bit.24480
This article evaluates the use of cation exchange chromatography as an alternative capture method for monoclonal antibodies. Using a model IgG4 cell culture harvest, the authors define a reasonable but limited range of conditions that provide acceptable values for yield, purity, and viral clearance. As shown in the accompanying figure, post-elution antibody integrity was assessed, revealing that appropriate levels of percent dimer (A) monomer (B) and half-antibody (C) forms were achieved.