Protein crystallization in stirred systems—scale-up via the maximum local energy dissipation (pages 1956–1963)
Benjamin Smejkal, Bernhard Helk, Jean-Michel Rondeau, Sabine Anton, Angelika Wilke, Peter Scheyerer, Jacqueline Fries, Dariusch Hekmat and Dirk Weuster-Botz
Article first published online: 9 FEB 2013 | DOI: 10.1002/bit.24845
Technical-scale protein crystallization in stirred vessels can be a viable alternative to purification as well as polishing via preparative chromatography, particularly for therapeutic proteins. However, little systematic know-how exists. In this study, scale-up of protein crystallization was investigated using a Fab-fragment of the therapeutic antibody Canakinumab and lysozyme. A faster onset of crystallization was observed at an optimum stirrer speed and a comparable crystallization behavior was achieved by applying a constant maximum local energy dissipation for scale-up.