Nanomechanics controls neuronal precursors adhesion and differentiation (pages 2301–2310)
Elisa Migliorini, Jelena Ban, Gianluca Grenci, Laura Andolfi, Alessandro Pozzato, Massimo Tormen, Vincent Torre and Marco Lazzarino
Article first published online: 16 MAR 2013 | DOI: 10.1002/bit.24880
The effect of substrate elasticity on neuronal precursors differentiation and adhesion is described. Top: picture which represents the deformations of soft (left) and rigid (right) substrates induced by the detachment of a neuronal precursors. Bottom: IF representative images of ES-derived cell culture on the two substrates with the corresponding neuronal yield; neurons were labeled with Tuj1 (green), neuronal precursors with nestin (red) and nuclei were stained with Hoechst. The neuronal yield was calculated 48 h after neuronal plating.