Beyond growth rate 0.6: What drives Corynebacterium glutamicum to higher growth rates in defined medium (pages 359–371)
Simon Unthan, Alexander Grünberger, Jan van Ooyen, Jochem Gätgens, Johanna Heinrich, Nicole Paczia, Wolfgang Wiechert, Dietrich Kohlheyer and Stephan Noack
Version of Record online: 24 SEP 2013 | DOI: 10.1002/bit.25103
The amino acid producer Corynebacterium glutamicum was cultivated in bioreactors in well-known CGXII medium at low inoculum (OD ≈ 0.005). Interestingly, in this highly diluted environment a growth rate of was observed, reproducing the authors' former results from microfluidic single cell cultivations (MSCC). Advanced growth studies, combining bioreactor and MSCC experiments, revealed a hidden co-substrate within CGXII. Protocatechuic acid (PCA), originally classified as iron chelator, is co-metabolized next to glucose and significantly accelerates growth by direct TCA feed.