Transcriptome dynamics of transgene amplification in Chinese hamster ovary cells (pages 518–528)
Nandita Vishwanathan, Huong Le, Nitya M. Jacob, Yung-Shyeng Tsao, Sze-Wai Ng, Bernard Loo, Zhong Liu, Anne Kantardjieff and Wei-Shou Hu
Article first published online: 18 OCT 2013 | DOI: 10.1002/bit.25117
Transcriptome analysis was performed on CHO cells undergoing selection and amplification of transgene and also on cell clones of high and low productivity. Amplification results in a moderate increase in transgene transcript level, but wide spread alterations in transcriptome. Many genes transcriptionally altered during cell line development are common to those differentially expressed between high and low producing cells. The consensus genes among conditions giving rise to high productivity, may constitute a hyper-productivity gene set and be used to facilitate cell line development.