Cell line profiling to improve monoclonal antibody production (pages 748–760)
Sohye Kang, Da Ren, Gang Xiao, Kristi Daris, Lynette Buck, Atim A. Enyenihi, Roman Zubarev, Pavel V. Bondarenko and Rohini Deshpande
Article first published online: 19 NOV 2013 | DOI: 10.1002/bit.25141
Inherent, genetic features of various monoclonal antibody-producing CHO cell lines were examined using microarray-based transcriptomics and LC-MS/MS shotgun proteomics approaches. Specific transcripts and proteins correlating with productivity, proliferation rate and cell size have been identified. Global structures of the expression profiles were also analyzed and compared in order to assess their contribution toward specific culture phenotype.