Disruption of the gene C12orf35 leads to increased productivities in recombinant CHO cell lines (pages 2433–2442)
Anett Ritter, Tatjana Rauschert, Mevion Oertli, Daniel Piehlmaier, Panagiotis Mantas, Genevieve Kuntzelmann, Nadine Lageyre, Barbara Brannetti, Bernd Voedisch, Sabine Geisse, Thomas Jostock and Holger Laux
Version of Record online: 14 JUN 2016 | DOI: 10.1002/bit.26009
Ritter and coworkers identified that the loss or reduced expression of the gene C12orf35 leads to increased mRNA levels of exogenes in CHO cells. Knock-out of C12orf35 in CHO cells results in a shorter recovery time during selection pressure and to significant higher productivity. This highlights that C12orf35 is negatively affecting the expression of recombinant proteins and is therefore a relevant gene for cell line engineering approaches.