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Hydrogen sulphide ameliorates ischaemia–reperfusion injury in an experimental model of non-heart-beating donor kidney transplantation

Authors

  • S. A. Hosgood,

    1. Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester LE5 4PW, UK
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  • M. L. Nicholson

    Corresponding author
    1. Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester LE5 4PW, UK
    • Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester LE5 4PW, UK
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  • Presented to meetings of the Society of Academic and Research Surgery, Bristol, UK, January 2009, the British Transplant Society, Liverpool, UK, April 2009, the Association of Surgeons of Great Britain and Ireland, Glasgow, UK, May 2009, and the European Society for Organ Transplantation Congress, Paris, France, August 2009; and published in abstract form as Br J Surg 2009; 95(Suppl 2): 2 and Transpl Int 2009; 22(Suppl 2): 44

Abstract

Background:

Therapies to alleviate ischaemia–reperfusion (IR) injury have an important role in kidney transplantation. This study used a porcine model of non-heart-beating (NHB) donor kidneys to investigate the effects of hydrogen sulphide on IR injury.

Methods:

Porcine kidneys were subjected to 25 min of warm ischaemia and 18 h of cold storage. They were reperfused ex vivo with autologous oxygenated blood to assess renal function. A group treated with hydrogen sulphide (0·5 mmol/l) infused 10 min before and after reperfusion (n = 6) was compared with an untreated control group (n = 7).

Results:

Hydrogen sulphide significantly improved renal blood flow compared with control values (mean(s.d.) area under the curve (AUC) 614·9(165·5) versus 270·3(86·7) ml per min per 100 g.h; P = 0·001) and renal function (AUC creatinine: 1640(248) versus 2328(154) µmol/l.h; P = 0·001; AUC creatinine clearance: 6·94(5·03) versus 0·96(0·32) ml per min per 100 g.h; P = 0·004). Oxidative damage was also reduced by hydrogen sulphide (urinary 8-isoprostane at 1 h of reperfusion: 478·9(237·1) versus 1605·6(632·7) pg/ml per mmol/l creatinine; P = 0·032).

Conclusion:

Hydrogen sulphide ameliorated the renal dysfunction associated with ischaemic damage, and has potential as a therapy against IR injury in NHB donor kidney transplantation. Copyright © 2009 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.

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