The most proficient enzyme as the central theme in an integrated, research-based biochemistry laboratory course

Authors

  • Jeffrey A. Smiley

    Corresponding author
    1. Department of Chemistry, Youngstown State University, Youngstown, Ohio 44555
    • Dept. of Chemistry, Youngstown State University, One University Plaza, Youngstown, OH. Tel.: 330-742-7103; Fax: 330-742-1579
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  • Course development was supported by equipment funds from the Youngstown State University Dean's Advisory Council and in part by a grant (to J. A. S.) from the American Chemical Society Petroleum Research Fund.

Abstract

The enzyme orotidine-5′-monophosphate decarboxylase is an attractive choice for the central theme of an integrated, research-based biochemistry laboratory course. A series of laboratory exercises common to most instructional laboratories, including enzyme assays, protein purification, enzymatic characterization, elementary kinetics, and recombinant DNA methods, has been devised. The series of exercises requires biochemical instrumentation common to most laboratories and some specialized materials available from the author's institution to laboratory instructors.

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