Detection of genetically modified organisms in food by DNA extraction and PCR amplification
Article first published online: 3 NOV 2006
Copyright © 2002 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 30, Issue 1, pages 51–55, January 2002
How to Cite
Thion, L., Vossen, C., Couderc, B., Erard, M. and Clemençon, B. (2002), Detection of genetically modified organisms in food by DNA extraction and PCR amplification. Biochem. Mol. Biol. Educ., 30: 51–55. doi: 10.1002/bmb.2002.494030010037
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Received: 14 DEC 2001
A practical class experiment based on the extraction/purification of DNA from soybeans and the identification of exogenous DNA sequences amplified by PCR is described. This is used in a biochemistry and molecular biology course for advanced science undergraduate students. Students are given small amounts of soybean powder, and they practice a DNA extraction, purification, and a PCR with a set of different specific primers followed by an electrophoresis to determine whether the sample is transgenic. A major aim is to provide experience in the use of common techniques such as DNA extraction/purification and PCR and their interpretation.