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Keywords:

  • Laboratory instruction;
  • biophysical chemistry;
  • fluorescence;
  • protein folding;
  • thermodynamics

Abstract

An undergraduate laboratory experiment to measure the free energy of denaturation of the enzyme lysozyme is described. The fluorescence of the protein in solutions of varying concentrations of urea and guanidine hydrochloride is measured. The data are analyzed graphically assuming a two-state model for the denaturation.