This work was supported by the National Science Foundation (CHE 9820176) and the Robert A. Welch Foundation (W-0031).
The free energy of denaturation of lysozyme: An undergraduate experiment in biophysical chemistry*
Article first published online: 3 NOV 2006
Copyright © 2002 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 30, Issue 4, pages 244–247, July 2002
How to Cite
Kurtin, W. E. and Lee, J. M. (2002), The free energy of denaturation of lysozyme: An undergraduate experiment in biophysical chemistry. Biochem. Mol. Biol. Educ., 30: 244–247. doi: 10.1002/bmb.2002.494030040083
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 7 MAR 2002
- Manuscript Received: 28 NOV 2001
- Laboratory instruction;
- biophysical chemistry;
- protein folding;
An undergraduate laboratory experiment to measure the free energy of denaturation of the enzyme lysozyme is described. The fluorescence of the protein in solutions of varying concentrations of urea and guanidine hydrochloride is measured. The data are analyzed graphically assuming a two-state model for the denaturation.