The free energy of denaturation of lysozyme: An undergraduate experiment in biophysical chemistry*


  • *

    This work was supported by the National Science Foundation (CHE 9820176) and the Robert A. Welch Foundation (W-0031).


An undergraduate laboratory experiment to measure the free energy of denaturation of the enzyme lysozyme is described. The fluorescence of the protein in solutions of varying concentrations of urea and guanidine hydrochloride is measured. The data are analyzed graphically assuming a two-state model for the denaturation.