The abbreviations used are: HEW, hen egg white; (NAG)3, chitotriose, (N-acetyl glucosamine)3; ITC, isothermal titration calorimetry.
Advanced instrumentation projects for first-year biochemistry laboratory†
Article first published online: 3 NOV 2006
Copyright © 2003 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 31, Issue 2, pages 115–118, March 2003
How to Cite
Hall, M. L., Guth, C. A., Kohler, S. J. and Wolfson, A. J. (2003), Advanced instrumentation projects for first-year biochemistry laboratory. Biochem. Mol. Biol. Educ., 31: 115–118. doi: 10.1002/bmb.2003.494031020194
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 21 NOV 2002
- Manuscript Received: 23 OCT 2002
- isothermal titration calorimetry;
Our first-semester biochemistry course is accompanied by a project-based laboratory with a focus on the enzyme lysozyme. The first half of the semester involves purification of the enzyme. In the second half, students learn to work with advanced instrumentation techniques, specifically fluorescence spectroscopy and isothermal titration calorimetry. These methods are linked, as much as possible, to the theme of lysozyme: its structure, stability, or binding of ligand. As a final project, students design an experiment involving one of these methods. Examples are determination of the pKa of the catalytic residue and the binding affinity for an inhibitor under different conditions of pH and temperature. Four weeks of laboratory are sufficient for students to learn how to use the instruments and to develop a short project. At the end of the project, students give an oral presentation on the theory of the method and their results and prepare a paper that undergoes peer review. Hands-on experience with these methods reinforces theoretical concepts taught in the lecture portion of the course.