DNA isolation from a dried blood sample, PCR amplification, and population analysis: Making the most of commercially available kits
Article first published online: 3 NOV 2006
Copyright © 2003 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 31, Issue 6, pages 418–421, November 2003
How to Cite
DeLong Frost, L. and Peart, S. T. (2003), DNA isolation from a dried blood sample, PCR amplification, and population analysis: Making the most of commercially available kits. Biochem. Mol. Biol. Educ., 31: 418–421. doi: 10.1002/bmb.2003.494031060268
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 24 JUN 2003
- Manuscript Received: 12 SEP 2002
- DNA extraction;
- DNA gel electrophoresis;
- Alu repeat;
- Hardy-Weinberg statistics
A 3-week undergraduate molecular biology laboratory exercise combining commercially available kits is described. The laboratory involves DNA extraction from a human dried blood sample, amplification of an Alu-repeat sequence found on chromosome 8, and analysis of the genetic and allelic frequency in the class population using Hardy-Weinberg statistics. The use of the prepared kits provides a means for the instructor of an undergraduate biochemistry course to explore molecular biology techniques on a limited budget and with a minimal time investment.