A simple method for detecting genetically modified maize in common food products
Article first published online: 3 NOV 2006
Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 32, Issue 1, pages 35–38, January 2004
How to Cite
Brinegar, C. and Levee, D. (2004), A simple method for detecting genetically modified maize in common food products. Biochem. Mol. Biol. Educ., 32: 35–38. doi: 10.1002/bmb.2004.494032010304
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Received: 12 AUG 2003
- Bt-toxin gene;
- maize invertase gene
A commercially available leaf DNA extraction and amplification kit has been adapted for the detection of genetically modified material in common food products containing maize. Amplification using published primer pairs specific for the Bacillus thuringiensis delta-endotoxin and maize invertase genes results in a 226-bp invertase PCR product in all samples (an internal positive control) plus a 184-bp product in samples that are genetically modified with the endotoxin gene. The ease and rapidity of DNA extraction and PCR make this exercise especially suitable for advanced-placement high school or lower division college biology students.