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This work was supported by Ministerio de Ciencia y Tecnología, Spain (BIO2003-07672).
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Contribution of each complex of the mitochondrial respiratory chain in the generation of the proton-motive force†
Article first published online: 3 NOV 2006
DOI: 10.1002/bmb.2004.494032010308
Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.
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How to Cite
Garcia-Vallve, S. (2004), Contribution of each complex of the mitochondrial respiratory chain in the generation of the proton-motive force. Biochem. Mol. Biol. Educ., 32: 17–19. doi: 10.1002/bmb.2004.494032010308
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Publication History
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 22 OCT 2003
- Manuscript Received: 25 APR 2003
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Keywords:
- Bioenergetics;
- mitochondrial respiratory chain;
- H+/2e− stoichiometry;
- proton-motive force
Abstract
A new parameter is presented for considering the contribution from each complex of the mitochondrial respiratory chain to the creation of the electrochemical gradient. This parameter is the proton gradient generated in each complex per pair of electrons transported (ΔH+/2e−) and may be calculated as the sum of the electrons taken up from the N-side and the electrons released at the P-side. The ΔH+/2e− values take into account all the electrons taken up and released in each complex and differentiate between the contribution from protons that are translocated from the N-side to the P-side and the contribution of scalar protons. Using these values, the P/O ratios for the oxidation of NADH and succinate are predicted to be 2.5 and 1.5, respectively.