A spectroscopic-based laboratory experiment for protein conformational studies*

Authors

  • Carlos Henrique I. Ramos

    Corresponding author
    1. Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, CP 6192, Campinas SP, 13084-971 Brazil, and Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas SP, Brazil
    • Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, CP 6192, Campinas SP, 13084-971 Brazil
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  • *

    This work was supported by the PEW Charitable Trusts, Fundaçao de Amparo à Pesquisa do Estado de Sao Paulo, and Conselho Nacional de Pesquisas/Ministerio da Ciencia e Technologia.

Abstract

This article describes a practical experiment for teaching basic spectroscopic techniques to introduce the topic of protein conformational change to students in the field of molecular biology, biochemistry, or structural biology. The spectroscopic methods employed in the experiment are absorbance, for protein concentration measurements, and fluorescence and circular dichroism, for probing protein conformational changes.

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