The zymogen-enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage*

Authors

  • João M. Pizauro Junior,

    Corresponding author
    1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil
    • Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil
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  • Jesus A. Ferro,

    1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil
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  • Andréa C. F. de Lima,

    1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil
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  • Karina S. Routman,

    1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil
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  • Maria Célia Portella

    1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil
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  • *

    This work was supported in part by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). A. C. F. L. was recipient of a scholarship from FAPESP; K. S. R. was recipient of a scholarship from CNPq; M. C. P., J. M. P., and J. A. F. were CNPq-supported Research Fellows.

Abstract

The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly “switched on” by specific proteolytic cleavage.

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