This work was supported in part by National Science Foundation Grant MCB-9876212 and the Florida Agricultural Experiment Station. This article is Florida Agricultural Experiment Station Journal Series Number R-09889.
Sex determination using PCR†
Article first published online: 3 NOV 2006
Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 32, Issue 2, pages 115–119, March 2004
How to Cite
Kima, P. E. and Rasche, M. E. (2004), Sex determination using PCR. Biochem. Mol. Biol. Educ., 32: 115–119. doi: 10.1002/bmb.2004.494032020327
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 24 OCT 2003
- Manuscript Received: 8 SEP 2003
- Agarose gel electrophoresis;
- DNA isolation
PCR has revolutionized many aspects of biochemistry and molecular biology research. In the following exercise, students learn PCR by isolating their own DNA, amplifying specific segments of the X and Y chromosomes, and estimating the sizes of the PCR products using agarose gel electrophoresis. Based on the pattern of PCR products, students can distinguish between male and female samples and determine the gender of an unknown DNA donor. The exercise is presented for upper division undergraduate majors in microbiology, biochemistry, and molecular biology, but can be adapted to different academic levels and disciplines. The use of student samples in the exercise can enhance learning of these techniques by making PCR and agarose gel electrophoresis directly relevant to the students.