Tagging and purifying proteins to teach molecular biology and advanced biochemistry
Article first published online: 3 NOV 2006
Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 32, Issue 6, pages 373–377, November 2004
How to Cite
Roecklein-Canfield, J. A. and Lopilato, J. (2004), Tagging and purifying proteins to teach molecular biology and advanced biochemistry. Biochem. Mol. Biol. Educ., 32: 373–377. doi: 10.1002/bmb.2004.494032060399
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 22 JUL 2004
- Manuscript Received: 2 DEC 2003
- Protein purification;
- His tagging;
- GST fusion proteins
Two distinct courses, “Molecular Biology” taught by the Biology Department and “Advanced Biochemistry” taught by the Chemistry Department, complement each other and, when taught in a coordinated and integrated way, can enhance student learning and understanding of complex material. “Molecular Biology” is a comprehensive lecture-based course with a 3-h laboratory once a week, while “Advanced Biochemistry” is a completely laboratory-based course with lecture fully integrated around independent student projects. Both courses emphasize and utilize cutting-edge technology. Teaching across departmental boundaries allows students access to faculty expertise and techniques rarely used at the undergraduate level, namely the tagging of proteins and their use in protein purification.