Screening a library of household substances for inhibitors of phosphatases: An introduction to high-throughput screening
Article first published online: 3 NOV 2006
Copyright © 2005 International Union of Biochemistry and Molecular Biology, Inc.
Biochemistry and Molecular Biology Education
Volume 33, Issue 1, pages 16–21, January 2005
How to Cite
Taylor, A. T. S. (2005), Screening a library of household substances for inhibitors of phosphatases: An introduction to high-throughput screening. Biochem. Mol. Biol. Educ., 33: 16–21. doi: 10.1002/bmb.2005.494033010430
- Issue published online: 3 NOV 2006
- Article first published online: 3 NOV 2006
- Manuscript Revised: 18 OCT 2004
- Manuscript Received: 12 JUL 2004
- Enzyme kinetics;
- enzyme inhibition;
- library screening;
- high-throughput screening;
- wheat germ acid phosphatase
Library screening methods are commonly used in industry and research. This article describes an experiment that screens a library of household substances for properties that would make a good “drug,” including enzyme inhibition, neutral pH, and nondenaturing to proteins, using wheat germ acid phosphatase as the target protein. An adaptation of the experiment appropriate for lower level biochemistry or outreach is also described.
This work was supported by Wabash College through the Haines Fund for the Study of Biochemistry and the National Science Foundation through Grant DUE 0126242.