PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
Article first published online: 7 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Biochemistry and Molecular Biology Education
Volume 40, Issue 2, pages 108–111, March/April 2012
How to Cite
Sorroche, F. G. and Giordano, W. (2012), PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti. Biochem. Mol. Biol. Educ., 40: 108–111. doi: 10.1002/bmb.20560
- Issue published online: 14 MAR 2012
- Article first published online: 7 DEC 2011
- Manuscript Revised: 7 SEP 2011
- Manuscript Received: 26 JUN 2011
- expR gen;
Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen-fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low-molecular-weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non-functional expR loci in S. meliloti.