Problem-solving test: Nucleocytoplasmic shuttling of pre-mrna binding proteins



Terms to be familiar with before you start to solve the test: transcription, pre-mRNA, RNA processing, RNA transport, RNA polymerase II, direct and indirect immunofluorescence staining, cell fractionation by centrifugation, oligo(dT)-cellulose chromatography, washing and elution of the column, ribonuclease, SDS-polyacrylamide gel electrophoresis, Western-blotting, pre-ribosomal particles, nuclear envelope, heterokaryon, phase contrast microscopy, polyadenylated RNA.