Problem-solving test: Digestion of a plasmid with restriction endonucleases


Address for correspondence to: József Szeberényi, Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. E-mail:


Terms to be familiar with before you start to solve the test: plasmid, restriction endonuclease, agarose gel electrophoresis, ethidium bromide staining, autoradiography, Coomassie staining, Southern blotting, linear and circular DNA, superhelical DNA, exonuclease, modification methylase, palindrome, sticky and blunt ends, nicked circular DNA. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(3):189–190, 2013