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This work was supported by the National Science Foundation (9977518).
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Laboratory Exercise
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A project-based biochemistry laboratory promoting the understanding and uses of fluorescence spectroscopy in the study of biomolecular structures and interactions†
Article first published online: 3 JUL 2007
DOI: 10.1002/bmb.56
Copyright © 2007 Wiley Periodicals, Inc.
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How to Cite
Briese, N. and Jakubowski, H. V. (2007), A project-based biochemistry laboratory promoting the understanding and uses of fluorescence spectroscopy in the study of biomolecular structures and interactions. Biochem. Mol. Biol. Educ., 35: 272–279. doi: 10.1002/bmb.56
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Publication History
- Issue published online: 11 JUL 2007
- Article first published online: 3 JUL 2007
- Manuscript Revised: 14 MAR 2007
- Manuscript Received: 18 JAN 2007
REFERENCES
- 1( 1995) Teaching biophysics: Approaches to teaching fluorescence spectroscopy. Biophys. J. 68, 1191–1195.
- 2,,,( 2003) Advanced instrumentation projects for first-year biochemistry laboratory. Biochem. Educ. 31, 115–118.
- 3,( 1997) Utilizing isolation, purification, and characterization of enzymes as project-oriented labs for undergraduate biochemistry. J. Chem. Educ. 74, 241–242.
- 4( 2006) A new project-based lab for undergraduate environmental and analytical chemistry. J. Chem. Educ. 83, 253–256.
- 5,,( 1996) Research in undergraduate instruction: A biotech lab project for recombinant DNA protein expression in bacteria. J. Chem. Educ. 73, 542–546.
- 6,,( 1996) An integrated biochemistry laboratory, including molecular modeling. J. Chem. Educ. 73, 1026–1029.
- 7,( 1996) Labeling histidines in cytochrome c: An integrated laboratory project. J. Chem. Educ. 73, 794–797.
- 8,,( 1998) Thermodynamic and kinetic controlled enolates: A project for a problem-oriented laboratory course. J. Chem. Educ. 75, 78–80.
- 9,( 2003) Labeling of proteins with fuorescent probes: Photophysical characterization of dansylated bovine serum albumin. Biochem. Educ. 31, 333–336.
- 10( 1999) A project-oriented biochemistry laboratory course. J. Chem. Educ. 76, 1130–1135.
- 11,,,( 2003) Development of a biochemistry laboratory course with a project-oriented goal. Biochem. Educ. 31, 106–112.
- 12,( 2002) Study of protein–ligand binding by fluorescence. Biochem. Educ. 30, 309–312.
- 13,( 2002) The free energy of denaturation of lysozyme: An undergraduate experiment in biophysical chemistry. Biochem. Educ. 30, 244–247.
- 14,( 2003) Determination of the molecular size of BSA by fluorescence anisotropy. Biochem. Educ. 31, 319–322.Direct Link:
- 15,( 2006) Fluorescence analysis of sulfonamide binding to carbonic anhydrase. Biochem. Educ. 34, 364–368.
- 16,( 2002) Protein tryptophan accessibility studied by fluorescence quenching. Biochem. Educ. 30, 175–178.
- 17,( 1993) Ribonuclease T1 and alcohol dehydrogenase fluorescence quenching by acrylamide: A laboratory experiment for undergraduate students. J. Chem. Educ. 70, 425–428.
- 18,( 1992) Probing denaturation by simultaneously monitoring residual enzyme activity and intrinsic fluorescence: An undergraduate biochemistry experiment. J. Chem. Educ. 69, 852–855.
- 19,,,,( 1996) Protein structure and chromatographic behavior: The separation and characterization of four proteins using gel filtration and ion-exchange chromatography and gel electrophoresis. J. Chem. Educ. 73, 268–272.
- 20( 1999) Principles of Fluorescence Spectroscopy, 2nd ed., Kluwer Academic/Plenum Publishers, New York.
- 21,,( 1993) Resolution of the fluorescence equilibrium unfolding profile of trp aporepressor using single tryptophan mutants. Protein Sci. 2, 1844–1852.
- 22,,,( 1990) Phase fluctuation in phospholipid membranes revealed by laurdan fluorescence. Biophys. J. 57, 1179–1186.
- 23,,,,,( 2003) Competitive binding of fatty acids and the fluorescent probe 1-8-anilinonaphthalene sulfonate to bovine β-lactoglobulin. Protein Sci. 12, 1596–1603.
- 24,,,( 1999) Defining the core structure of the α-lactalbumin molten globule state. J. Mol. Biol. 294, 213–221.
- 25,( 2003) Structural characterisation of the human α-lactalbumin molten globule at high temperature. J. Mol. Biol. 330, 1177–1188.
- 26,,( 1998) Monoacylglycerol binding to human serum albumin: Evidence that monooleoylglycerol binds at the dansylsarcosine site. J. Lip. Res. 39, 1033–1038.
- 27,,( 1998) Micellar aggregation numbers—A fluorescence study. J. Chem. Educ. 75, 93–98.
- 28,,( 2001) Fluorescence polarization as a tool to pinpoint vesicle thermal phase transitions. J. Chem. Educ. 78, 1100–1103.
- 29,,,,( 2005) On the difference in stability between horse and sperm whale myoglobins. Arch. Biochem. Biophys. 436, 168–177.
- 30,( 1998) 1-Anilino-8-naphthalene sulfonate anion-protein binding depends primarily on ion pair formation. Biophys. J. 74, 422–429.
- 31,, ( 1999) Automated flow injection gradient technique for binding studies of micromolecules to proteins using potentiometric sensors: Application to bovine serum albumin with anilinonaphthalenesulfonate probe and drugs. Anal. Chem. 71, 2541–2550.
- 32,,( 2000) Use of domain specific ligands to study urea-induced unfolding of bovine serum albumin. Biochem. Biophys. Res. Comm. 277, 83–88.