Liquid chromatographic method for the determination of sirolimus in blood using electrochemical detection
Article first published online: 23 SEP 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Volume 23, Issue 3, pages 267–272, March 2009
How to Cite
Mochizuki, N., Suka, E., Matsumoto, K., Akimoto, O., Ohno, K., Shimamura, T., Furukawa, H., Todo, S. and Kishino, S. (2009), Liquid chromatographic method for the determination of sirolimus in blood using electrochemical detection. Biomed. Chromatogr., 23: 267–272. doi: 10.1002/bmc.1109
- Issue published online: 9 FEB 2009
- Article first published online: 23 SEP 2008
- Manuscript Accepted: 18 JUN 2008
- Manuscript Revised: 11 JUN 2008
- Manuscript Received: 16 FEB 2008
- electrochemical detection
Therapeutic drug monitoring of sirolimus (rapamycin) is important for immunosuppressive therapy in solid organ transplantation. We have developed a simple and reliable method for determining blood concentrations of sirolimus using reversed-phase HPLC with electrochemical detection (ECD). The E2 potential was set at +900 mV. The potential of guard cell was set at +950 mV and that of the E1 cell at +400 mV. The method was linear for a concentration range of 1–50 ng/mL when 0.5 mL blood was used. The correlation coefficients of all standard curves were greater than or equal to 0.999. The limit of detection was 0.5 ng/mL. The inter-assay precision ranged from 3.22 to 7.48%, and the coefficient of variation (CV) for a quality control sample at 10 ng/mL was 7.48% with a bias of 8.4% from the target value. The intra-assay precision ranged from 0.72 to 3.71%, and the CV for a quality control sample at 10 ng/mL was 0.72% with a bias of 6.8% from the target value. In a solid organ transplant recipient, trough concentrations of sirolimus were well within the analytic range of the HPLC/ECD procedure. The method described here is suitable for management of sirolimus therapy in solid organ transplantation. Copyright © 2008 John Wiley & Sons, Ltd.