Get access

Improved chromatographic fingerprints for facile differentiation of two Ganoderma spp.

Authors

  • Chun-Mei Fu,

    1. Key Laboratory of Drug Targeting, West China School of Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China
    2. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, People's Republic of China
    Search for more papers by this author
  • Guang-Hua Lu,

    1. School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, People's Republic of China
    Search for more papers by this author
  • Oliver J. Schmitz,

    1. Department of Analytical Chemistry, University of Wuppertal, Gauss-Str. 20, 42119 Wuppertal, Germany
    Search for more papers by this author
  • Zhang-Wan Li,

    1. Key Laboratory of Drug Targeting, West China School of Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China
    Search for more papers by this author
  • Kelvin Sze-Yin Leung

    Corresponding author
    1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, People's Republic of China
    • Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, People's Republic of China.
    Search for more papers by this author

Abstract

This paper addresses a comprehensive and comparative study of six phytochemical extraction methods for triterpenes from the fruiting body of Ganoderma spp. Quantitative analysis of extracts was performed by HPLC with photodiode array detection. In general, pressurized liquid extraction and microwave-assisted extraction under optimized conditions produce better yields, and the former also significantly reduces the total time of extraction and manipulation of a sample, as well as the amount of solvent used in comparison with conventional soxhlet, reflux, ultrasonic, and methanol–CO2 supercritical fluid extractions. Based on the improved extraction protocol, the fingerprinting profiles for two species of Lingzhi were established using the consistent chromatographic features of 12 authentic samples. Eleven common peaks of ganoderic/ganoderenic acids were identified using LC-ESI-MS-MS. These specific triterpene groups were adopted as chemical markers for Lingzhi. Using chemometric analysis, the developed fingerprinting was successfully applied to differentiate between the two species under the Ganoderma genus and is applicable as a method for quality evaluation of this valuable medicinal fungus and its related proprietary products. Copyright © 2008 John Wiley & Sons, Ltd.

Ancillary