Abbreviations used: RSM, rivastigmine; TBME, tertiary methyl butyl ether.
Development and validation of a HPLC method for quantification of rivastigmine in rat urine and identification of a novel metabolite in urine by LC-MS/MS †
Article first published online: 10 JUN 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Volume 25, Issue 3, pages 353–361, March 2011
How to Cite
Arumugam, K., Chamallamudi, M. R., Gilibili, R. R., Mullangi, R., Ganesan, S., Kar, S. S., Averineni, R., Shavi, G. and Udupa, N. (2011), Development and validation of a HPLC method for quantification of rivastigmine in rat urine and identification of a novel metabolite in urine by LC-MS/MS . Biomed. Chromatogr., 25: 353–361. doi: 10.1002/bmc.1455
This article was published online on 10 June 2010. Errors were subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected [27 August 2010].
- Issue published online: 15 FEB 2011
- Article first published online: 10 JUN 2010
- Manuscript Accepted: 23 MAR 2010
- Manuscript Received: 8 FEB 2010
- rat urine;
A sensitive, specific and accurate HPLC method for the quantification of rivastigmine (RSM) in rat urine was developed and validated. The method involves the simple liquid–liquid extraction of RSM and pyridostigmine as an internal standard (IS) from rat urine with tertiary methyl butyl ether. The chromatographic separation of RSM and IS was achieved with 20 mm ammonium acetate buffer (pH 6.5) and acetonitrile (65:35, v/v) delivered at flow-rate of 1 mL/min on a Kromasil KR-100. The method was in linear range from 50 to 5000 ng/mL. The validation was done as per FDA guidelines and the results met the acceptance criteria. The method was successfully applied for the quantification of RSM in rat urine. Besides method validation, we have identified two metabolites of RSM in urine. Both the metabolites were characterized by HPLC-PDA and LC-MS/MS and it was found that one metabolite is novel. Copyright © 2010 John Wiley & Sons, Ltd.