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Detection and identification of diterpenoid alkaloids, isoflavonoids and saponins in Qifu decoction and rat plasma by liquid chromatography–time-of-flight mass spectrometry

Authors

  • Guangguo Tan,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, China
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    • Guangguo Tan and Jing Jing contributed equally to this work.
  • Jing Jing,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, China
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    • Guangguo Tan and Jing Jing contributed equally to this work.
  • Zhenyu Zhu,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, China
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  • Ziyang Lou,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China
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  • Wuhong Li,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, China
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  • Liang Zhao,

    1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, China
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  • Guoqing Zhang,

    1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, China
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  • Yifeng Chai

    Corresponding author
    1. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, China
    • Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China
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Yifeng Chai, Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China. E-mail: yfchai@smmu.edu.cn

ABSTRACT

A liquid chromatography–time-of-flight mass spectrometric (LC-TOFMS) method has been developed for analysis of components in Qifu decoction (QFD), a traditional Chinese medical formula consisting of Radix Astragali and Acontium carmichaeli, and in rat plasma after oral administration. Based on accurate mass measurements within 3 ppm error for each molecular ion and subsequent fragment ions of TOFMS, as well as matching of empirical molecular formulae with those of published components in the in-house chemical library, a total of 44 major components including 21 diterpenoid alkaloids, 12 flavonoids and 11 saponins were identified in QFD. After oral administration of QFD, 22 components in rat plasma were detected and identified by comparing and contrasting the constituents measured in QFD with those in the plasma samples. The results provided valuable chemical information for further pharmacology and active mechanism research on QFD. LC-TOFMS was also applied for the comparison of relative peak area of major active components between QFD and the single herb extracts. The concentration ratios of major saponins detected in the crude herb Radix Astragali were found to be different from those in QFD. The experimental data indicated that the decocting process could result in differences in the amounts of active components. Copyright © 2011 John Wiley & Sons, Ltd.

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