The first two authors have contributed equally.
Simultaneous isolation of artemisinin and its precursors from Artemisia annua L. by preparative RP-HPLC
Version of Record online: 19 SEP 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 6, pages 708–713, June 2012
How to Cite
Tian, N., Li, J., Liu, S., Huang, J., Li, X. and Liu, Z. (2012), Simultaneous isolation of artemisinin and its precursors from Artemisia annua L. by preparative RP-HPLC. Biomed. Chromatogr., 26: 708–713. doi: 10.1002/bmc.1719
- Issue online: 1 MAY 2012
- Version of Record online: 19 SEP 2011
- Manuscript Accepted: 14 AUG 2011
- Manuscript Received: 22 JUN 2011
- National Science Foundation of China. Grant Number: 30700058
- Scientific Research Fund of Hunau Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop. Grant Number: 2009011
- Artemisia annua L;
- artemisinic acid;
- preparative HPLC
It is still a major challenge to simultaneously isolate artemisinin and its precursors, especially dihydroartemisinic acid and artemisinic acid, from herbal Artemisia annua. A rapid, economical and automatical chromatographic separation process to isolate and purify artemisinin, dihydroartemisinic acid and artemisinic acid at the same time on a preparative scale was developed. The procedure included solvent extraction of ground Artemisia annua leaves by refluxing and purification of crude extract by preparative reverse-phase high-performance liquid chromatography (RP-HPLC). Fractions containing artemisinin and its precursors were collected and identified by gas chromatography and mass spectrometry. High purity of artemisinin, dihydroartemisinic acid and artemisinic acid was obtained by preparative HPLC with a C18 column and 60% acetonitrile in water as the mobile phase. The techniques described here are useful tools for the preparative-scale isolation of artemisinin and its precursors in a fast, cost-effective and environmental friendly manner. Copyright © 2011 John Wiley & Sons, Ltd.