Rapid determination and comparative pharmacokinetics of tetrahydropalmatine in spontaneously hypertensive rats and normotensive rats

Authors

  • Zhanying Hong,

    Corresponding author
    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China
    • Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, People's Republic of China
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  • Guojun Cai,

    1. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China
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  • Wentao Ma,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, People's Republic of China
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  • Jun Wen,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, People's Republic of China
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  • Yifeng Chai,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China
    2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, People's Republic of China
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  • Guorong Fan

    Corresponding author
    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China
    • Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, People's Republic of China
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Z. Hong, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, People's Republic of China. E-mail: hongzhy001@yahoo.com.cn

G. Fan, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, People's Republic of China. E-mail: guorfan@yahoo.com.cn

ABSTRACT

A rapid high-performance liquid chromatographic method was developed and validated for determination of tetrahydropalmatine (THP), an active component of Rhizoma Corydalis, in rat plasma. The samples were prepared using protein precipitation and separated on an Agilent XDB-C18 column (150 × 4.6 mm, 5 µm) with the mobile phase consisting of methanol–0.1% phosphate acid solution, adjusted with triethylamine to pH 5.5 (65:35). Good linearity was found within 0.10–10.00 µg/mL of THP in rat plasma sample. The intra- and inter-day precision values were less than 10%. The developed method was successfully applied to assess the pharmacokinetics of THP in spontaneously hypertensive rats (SHR) and normotensive rats. After oral administration of a single dose of THP (60 mg/kg), the maximum plasma concentrations were 6.15 ± 2.1 and 7.54 ± 2.9 µg/mL for normotensive rats and SHR, respectively. The mean values of AUC0–∞ of THP in SHR were 81.44 ± 45.0 µg h/mL, significantly higher (p < 0.05) than in normotensive rats (44.06 ± 19.6 µg h/mL). The t1/2 and MRT in SHR were much longer than that in healthy Sprague–Dawley rats, indicating slow elimination of THP in SHR. The results indicated that there are some differences in pharmacokinetics of THP in SHR and Sprague–Dawley rats and it is very important to investigate the pharmacokinetic properties of drugs in pathological conditions. Copyright © 2011 John Wiley & Sons, Ltd.

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