HPLC-UV analytical method for determination of pizotifen after in vitro transdermal diffusion studies
Article first published online: 17 OCT 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 6, pages 769–774, June 2012
How to Cite
Serna-Jiménez, C. E., del Rio Sancho, S., Calatayud-Pascual, M. A., Balaguer-Fernández, C., Femenía-Font, A., López-Castellano, A. and Merino, V. (2012), HPLC-UV analytical method for determination of pizotifen after in vitro transdermal diffusion studies. Biomed. Chromatogr., 26: 769–774. doi: 10.1002/bmc.1727
- Issue published online: 1 MAY 2012
- Article first published online: 17 OCT 2011
- Manuscript Accepted: 11 SEP 2011
- Manuscript Received: 13 JUL 2011
- Fondo de Investigaciones Sanitarias. Grant Number: PI060108
- Universidad CEU Cardenal Herrera. Grant Number: PRCEU-UCH01/09
- pizotifen malate;
- transdermal diffusion;
- high-performance liquid chromatography;
Pizotifen malate is an antihistamine and serotonin inhibitor used in the preventive treatment of migraine and eating disorders. A simple, rapid, accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection was validated for the quantitative analysis of pizotifen malate in samples from in vitro transdermal diffusion studies. The method was validated for specificity, linearity, accuracy, precision, limit of detection, limit of quantification and robustness. Drug stability in the solution was also determined under different conditions. Separation was carried out using a 250 × 4.0 mm Kromasil® C18 column at room temperature. The detector response, fitted at 254 nm, was found to be linear in a concentration range between 0.24 and 24.0 µg/mL. The limit of detection was 0.02 µg/mL and the limit of quantification was 0.07 µg/mL. Finally, in vitro transdermal diffusion of pizotifen malate was characterized using the validated HPLC method. Copyright © 2011 John Wiley & Sons, Ltd.