In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn
Article first published online: 20 APR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 1, pages 96–101, January 2013
How to Cite
Qi, P., Fan, M., Li, Z., Chen, M., Sun, Z., Wu, B. and Huang, C. (2013), In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn. Biomed. Chromatogr., 27: 96–101. doi: 10.1002/bmc.2753
- Issue published online: 13 DEC 2012
- Article first published online: 20 APR 2012
- Manuscript Accepted: 29 MAR 2012
- Manuscript Revised: 8 MAR 2012
- Manuscript Received: 9 FEB 2012
- National Science and Technology. Grant Numbers: 2009ZX09301-001, 2009ZX09308-005
- LTQ XL;
In order to illustrate the main biotransformation pathways of vaccarin in vivo, metabolites of vaccarin in rats were identified using a specific and sensitive high-performance liquid chromatography–electrospray ionization linear ion trap mass spectrometry (LTQ XL™) method. The rats were administered a single dose (200 mg/kg) of vaccarin by oral gavage. By comparing their changes in molecular masses (ΔM), retention times and spectral patterns with those of the parent drug, the parent compound and six metabolites were found in rat urine after oral administration of vaccarin. The parent compound and five metabolites were detected in rat plasma. In heart, liver and kidney samples, respectively, one, four and three metabolites were identified, in addition to the parent compound. Three metabolites, but no trace of parent drug, were found in the rat feces. This is the first systematic metabolism study of vaccarin in vivo. The biotransformation pathways of vaccarin involved methylation, hydroxylation, glycosylation and deglycosylation. Copyright © 2012 John Wiley & Sons, Ltd.