Preclinical pharmacokinetic analysis of armillarisin succinate ester in mouse plasma and tissues by LC–MS/MS
Article first published online: 5 JUN 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 1, pages 130–136, January 2013
How to Cite
Chen, Q., Li, P., Zhang, J. and Zhu, J. (2013), Preclinical pharmacokinetic analysis of armillarisin succinate ester in mouse plasma and tissues by LC–MS/MS. Biomed. Chromatogr., 27: 130–136. doi: 10.1002/bmc.2762
- Issue published online: 13 DEC 2012
- Article first published online: 5 JUN 2012
- Manuscript Accepted: 24 APR 2012
- Manuscript Revised: 7 APR 2012
- Manuscript Received: 11 FEB 2012
- liquid chromatography–mass spectrometry;
- armillarisin succinate ester;
- preclinical evaluation
A liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed and validated to determine the concentration of armillarisin succinate ester in mouse plasma and tissues, used for preclinical evaluation. Bavachin was employed as the internal standard. Separation was performed on a 3.5 µm Zorbax SB-C18 column (30 × 2.1 mm), with a mobile phase consisting of methanol and aqueous 20 mm ammonium acetate. Both analyte and internal standard were determined using electrospray ionization and the MS data acquisition was via selected ion monitoring in negative scanning mode. Quantification was performed using the transitions m/z 333 233 and 323 221 for armillarisin succinate ester and internal standard, respectively. The method was validated with respect to linearity, accuracy, precision, recovery and stability. This assay has been successfully applied to a pharmacokinetic and tissue distribution study after intravenous injection of ASE in mouse in a dose of 10 mg/kg. Copyright © 2012 John Wiley & Sons, Ltd.