Get access

Liquid chromatography and mass spectrometry of haem biosynthetic intermediates: a review


Chang Kee Lim, Clinical Biochemistry, King's College Hospital, Denmark Hill, London SE5 9RS, UK. E-mail:


This article discusses the separation, analysis and characterisation of intermediates and oxidative by-products of the haem biosynthetic pathway by liquid chromatography and mass spectrometry. Techniques reviewed include high-performance liquid chromatography, ultra-high-performance liquid chromatography, capillary electrophoresis, ion mobility spectrometry, mass spectrometry and tandem mass spectrometry. The emphasis was on the analysis of biological and clinical samples. Copyright © 2012 John Wiley & Sons, Ltd.