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Liquid chromatography and mass spectrometry of haem biosynthetic intermediates: a review

Authors


Chang Kee Lim, Clinical Biochemistry, King's College Hospital, Denmark Hill, London SE5 9RS, UK. E-mail: limchangkee@gmail.com

ABSTRACT

This article discusses the separation, analysis and characterisation of intermediates and oxidative by-products of the haem biosynthetic pathway by liquid chromatography and mass spectrometry. Techniques reviewed include high-performance liquid chromatography, ultra-high-performance liquid chromatography, capillary electrophoresis, ion mobility spectrometry, mass spectrometry and tandem mass spectrometry. The emphasis was on the analysis of biological and clinical samples. Copyright © 2012 John Wiley & Sons, Ltd.

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