Determination of the uronic acid composition of seaweed dietary ﬁbre by HPLC
Article first published online: 25 NOV 2003
Copyright © 2003 John Wiley & Sons, Ltd.
Volume 18, Issue 2, pages 90–97, March 2004
How to Cite
Sánchez-Machado, D. I., López-Cervantes, J., López-Hernández, J., Paseiro-Losada, P. and Simal-Lozano, J. (2004), Determination of the uronic acid composition of seaweed dietary ﬁbre by HPLC. Biomed. Chromatogr., 18: 90–97. doi: 10.1002/bmc.297
- Issue published online: 10 MAR 2004
- Article first published online: 25 NOV 2003
- Manuscript Accepted: 20 MAY 2003
- Manuscript Received: 1 MAY 2003
- Xunta de Galicia. Grant Number: PGIDITO2RMA20301PR
- edible seaweeds;
- β-d-mannuronic acid and α-l-guluronic acid ratio;
- ion-exchange chromatography;
A high-performance liquid chromatographic (HPLC) method is described for determination of the ratio of β-d-mannuronic acid to α-l-guluronic acid (M/G ratio) in dietary ﬁbre of edible seaweeds. Total dietary ﬁbre (TDF) content was determined gravimetrically. The TDF fraction was hydrolysed with 12 m and 1 m H2SO4, then neutralized with AG 4 × 4 resin. The uronic acids were separated in a Tracer Extrasil SAX 5 µm column (25 cm × 4 mm) at 35°C, with 2 mm KH2PO4 containing 5% methanol as mobile phase at a ﬂow rate of 1.5 mL/min. The detection wavelength was UV 210 nm. The chromatographic identiﬁcations of β-d-mannuronic acid and α-l-guluronic acid were conﬁrmed by liquid chromatography–mass spectrometry (LC-MS). The method precision was 1.4% for β-d-mannuronic acid and 3.5% for α-l-guluronic acid. The method was used to determine M/G ratio in canned seaweeds (Saccorhiza polyschides and Himanthalia elongata) and in dried seaweeds (H. elongata, Laminaria ochroleuca, Undaria pinnatiﬁda, Palmaria sp. and Porphyra sp.). Copyright © 2003 John Wiley & Sons, Ltd.