Research Article

An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

  1. Li Linxiang1,
  2. Yoshihiro Abe1,*,
  3. Yukiko Nagasawa1,
  4. Risa Kudo1,
  5. Noriko Usui1,
  6. Kazuhiro Imai1,
  7. Tadahiko Mashino1,
  8. Masataka Mochizuki1,
  9. Naoki Miyata2

Article first published online: 14 JAN 2004

DOI: 10.1002/bmc.339

Issue

Biomedical Chromatography

Biomedical Chromatography

Volume 18, Issue 7, pages 470–474, September 2004

Additional Information

How to Cite

Linxiang, L., Abe, Y., Nagasawa, Y., Kudo, R., Usui, N., Imai, K., Mashino, T., Mochizuki, M. and Miyata, N. (2004), An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. Biomedical Chromatography, 18: 470–474. doi: 10.1002/bmc.339

Author Information

  1. 1

    Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan

  2. 2

    Nagoya City University, Fucalty of Pharmaceutical Sciences, 3-1 Tanabe-dori, Mizuho, Nagoya 467-8603, Japan

*Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan.

Publication History

  1. Issue published online: 25 AUG 2004
  2. Article first published online: 14 JAN 2004
  3. Manuscript Revised: 18 SEP 2003
  4. Manuscript Accepted: 18 SEP 2003
  5. Manuscript Received: 24 JUL 2003

Funded by

  • Araki Grant.
  • Ministry of Health, Labor and Welfare, Japan.

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Keywords:

  • terephthalic acid;
  • 2-hydroxyterephthalic acid;
  • Fenton reaction;
  • hydroxyl radical;
  • HPLC;
  • fluorescence detection

Abstract

An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 mmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5–50 µmol/L, where the concentration of Fe(II) was 50 µmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol). Copyright © 2004 John Wiley & Sons, Ltd.

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