Simultaneous determination of albiﬂorin and paeoniﬂorin in rat urine by solid-phase extraction and high-performance liquid chromatography following oral administration of Si–Wu decoction
Article first published online: 2 JUL 2004
Copyright © 2004 John Wiley & Sons, Ltd.
Volume 18, Issue 10, pages 785–790, December 2004
How to Cite
Sheng, Y., Li, L., Zhang, J. and Guo, D. (2004), Simultaneous determination of albiﬂorin and paeoniﬂorin in rat urine by solid-phase extraction and high-performance liquid chromatography following oral administration of Si–Wu decoction. Biomed. Chromatogr., 18: 785–790. doi: 10.1002/bmc.389
- Issue published online: 3 DEC 2004
- Article first published online: 2 JUL 2004
- Manuscript Accepted: 8 MAR 2004
- Manuscript Revised: 10 FEB 2004
- Manuscript Received: 5 JAN 2004
- Ministry of Science and Technology of China. Grant Number: 2002BA906A29; 2002DEA 20021
- Commission of Science and Technology of Beijing
- solid-phase extraction;
- Si–Wu decoction
A high performance liquid chromatographic method (HPLC), together with solid phase extraction (SPE), was developed for simultaneous determination of albiﬂorin and paeoniﬂorin in rat urine after oral administration of Si–Wu decoction. The samples were pretreated with solid phase extraction using Extract-Clean™ cartridges. Analysis of the extract was performed on a reversed-phase C18 column and a mobile phase made up of acetonitrile and 0.03% formic acid (17:83, v/v). UV detection was set at 230 nm. The assay was linear over the range 2.625–52.50 mg/mL for albiﬂorin and 3.875–77.50 µg/mL for paeoniﬂorin. The average percentage recoveries of three spiked urines were 97.01 ± 3.32 and 102.32 ± 6.97 for albiﬂorin and paeoniﬂorin, respectively. The intra-day precision (RSD) ranged from 0.21 to 1.79% at concentrations of 4.20, 10.50, 26.25 and 39.375 µg/mL of albiﬂorin and 0.12 to 2.92% at concentrations of 3.875, 10.85, 23.25 and 58.125 µg/mL of paeoniﬂorin, and inter-day precision (RSD) was from 1.02 to 1.86% for albiﬂorin and 0.94 to 3.30% for paeoniﬂorin, at the same four concentrations. This method was applied in order to analyze albiﬂorin and paeoniﬂorin in rat urine following oral administration of traditional Chinese medicinal preparation of Si–Wu decoction. Copyright © 2004 John Wiley & Sons, Ltd.