Analytical method of chondroitin/dermatan sulfates using high performance liquid chromatography/turbo ionspray ionization mass spectrometry: application to analyses of the tumor tissue sections on glass slides
Article first published online: 10 AUG 2001
Copyright © 2001 John Wiley & Sons, Ltd.
Volume 15, Issue 5, pages 356–362, August 2001
How to Cite
Oguma, T., Toyoda, H., Toida, T. and Imanari, T. (2001), Analytical method of chondroitin/dermatan sulfates using high performance liquid chromatography/turbo ionspray ionization mass spectrometry: application to analyses of the tumor tissue sections on glass slides. Biomed. Chromatogr., 15: 356–362. doi: 10.1002/bmc.74
- Issue published online: 10 AUG 2001
- Article first published online: 10 AUG 2001
- Manuscript Accepted: 27 SEP 2000
- Manuscript Revised: 26 SEP 2000
- Manuscript Received: 11 AUG 2000
We established a highly sensitive quantitative analytical method for chondroitin/dermatan sulfates by LC/MS method. By this method, the unsaturated disaccharides produced after the enzymatic digestion of chondroitin/dermatan sulfates can be determined in the amounts as low as 0.5 pmol levels. The use of tetrabutylammonium hydroxide as an ion-pair reagent for LC/MS allowed us to separate unsaturated 4-sulfated disaccharide and unsaturated 6-sulfated disaccharide. Furthermore, the peak areas of unsaturated disaccharides were increased almost 10 times by the postcolumn addition of acetonitrile. We applied this LC/MS method to the analyses of unsaturated disaccharides from chondroitin/dermatan sulfates in the tissues sections on glass slides, which were prepared from MethA tumor-bearing mice. This method brought about considerable reduction in the time distance from sample collection to preparation of analytical results. Copyright © 2001 John Wiley & Sons, Ltd.
2-acetamido-2-deoxy-3-O-(β-D-gluco-4-enopyranosyluronic acid)-6-O-sulfo-D-galactose, ΔDiHS-NS, 2-deoxy-2-sulfamino-4-O-(4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid)-D-glucose