High-performance liquid chromatography analysis of curcumin in rat plasma: application to pharmacokinetics of polymeric micellar formulation of curcumin
Article first published online: 5 MAR 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Volume 21, Issue 5, pages 546–552, May 2007
How to Cite
Ma, Z., Shayeganpour, A., Brocks, D. R., Lavasanifar, A. and Samuel, J. (2007), High-performance liquid chromatography analysis of curcumin in rat plasma: application to pharmacokinetics of polymeric micellar formulation of curcumin. Biomed. Chromatogr., 21: 546–552. doi: 10.1002/bmc.795
- Issue published online: 17 APR 2007
- Article first published online: 5 MAR 2007
- Manuscript Accepted: 12 DEC 2006
- Manuscript Revised: 8 DEC 2006
- Manuscript Received: 20 NOV 2006
- Natural Sciences and Engineering Research Council of Canada. Grant Number: STPGP 336986
- Canadian Institute of Health Research. Grant Number: MOP 42407
- polymeric micelle;
A simple, rapid and reliable high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of curcumin in rat plasma. Plasma was precipitated with acetonitrile after addition of the internal standard (IS), 4-hydroxybenzophenone. Separation was achieved on a Waters µBondapak™ C18 column (3.9 × 300 mm, 5 µm) using acetonitrile (55%) and citric buffer, pH 3.0 (45%) as the mobile phase (flow rate = 1.0 mL/min). The UV detection wavelength was 300 and 428 nm for IS and curcumin, respectively. The extraction efficiencies were 97.08, 95.69 and 94.90% for 50, 200 and 1000 ng/mL of curcumin in rat plasma, respectively. The calibration curve was linear over the range 0.02–1 µg/mL with a correlation coefficient of r2 > 0.999. The intra- and inter-day coefficients of variation were less than 13%, and mean intra- and inter-day errors were less than ±6% at 50, 200 and 1000 ng/mL of curcumin. This assay was successfully applied to the pharmacokinetic studies of both solubilized curcumin and its polymeric micellar formulation in rats. It was found that polymeric micelles increased the half-life of curcumin 162-fold that of solubilized curcumin and increased the volume of distribution (Vdss) by 70-fold. Copyright © 2007 John Wiley & Sons, Ltd.