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Characterization of constituents in Stellera chamaejasme L. by rapid-resolution liquid chromatography–diode array detection and electrospray ionization time-of-flight mass spectrometry

Authors

  • Liang Zhao,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
    2. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, People's Republic of China
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  • Zi-Yang Lou,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
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  • Zhen-Yu Zhu,

    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
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  • Hai-Zhang,

    1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, People's Republic of China
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  • Guo-Qing Zhang,

    1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, People's Republic of China
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  • Yi-Feng Chai

    Corresponding author
    1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
    • Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China.
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Abstract

A reliable and rapid method based on rapid-resolution liquid chromatography–diode array detection (RRLC-DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) has been developed for the isolation and characterization of multiple constituents in the root of Stellera chamaejasme L., which was extracted by sonication with methanol in an optimized procedure. Separation of the multiple constituents was achieved on an Agilent Zorbax XDB-C18 (50 × 3.0 mm i.d.; 1.8 µm) column using a gradient elution at a flow rate of 0.4 mL/min. The detection wavelength was 210 nm. Mass spectra were acquired in both positive and negative modes. A formula database of the known chemical constituents in the root of Stellera chamaejasme L. was established by an Agilent software. Twenty-two obvious peaks appeared in the total ion chromatogram and nine of them were characterized by TOF/MS. The RRLC-DAD and ESI-TOF/MS method with ultrasonic extraction would be useful for rapid and effective characterization of chemical constituents in the root of Stellera chamaejasme L. Copyright © 2007 John Wiley & Sons, Ltd.

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