Analysis of cell cultures of Taxus wallichiana using direct analysis in real-time mass spectrometric technique
Article first published online: 15 OCT 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Volume 22, Issue 3, pages 250–253, March 2008
How to Cite
Banerjee, S., Madhusudanan, K. P., Khanuja, S. P. S. and Chattopadhyay, S. K. (2008), Analysis of cell cultures of Taxus wallichiana using direct analysis in real-time mass spectrometric technique. Biomed. Chromatogr., 22: 250–253. doi: 10.1002/bmc.919
- Issue published online: 4 MAR 2008
- Article first published online: 15 OCT 2007
- Manuscript Accepted: 2 AUG 2007
- Manuscript Received: 27 JUL 2007
- DART MS;
- Taxus wallichiana
The applicability of a new mass spectrometric technique, DART (direct analysis in real time) has been studied in the analysis of the calli of Taxus wallichiana. The intact callus samples were directly analyzed by holding them in the gap between the DART source and mass spectrometer for measurements. Five C-14 oxygenated taxoids were characterized from the analysis of the calli of the Taxus wallichiana almost instantaneously. The confirmation of the structures of the identified taxoids was made through their accurate molecular formula determinations. Copyright © 2007 John Wiley & Sons, Ltd.