Expression of tropane alkaloids in the hairy root culture of Atropa acuminata substantiated by DART mass spectrometric technique
Version of Record online: 2 APR 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Volume 22, Issue 8, pages 830–834, August 2008
How to Cite
Banerjee, S., Madhusudanan, K. P., Chattopadhyay, S. K., Rahman, L. U. and Khanuja, S. P. S. (2008), Expression of tropane alkaloids in the hairy root culture of Atropa acuminata substantiated by DART mass spectrometric technique. Biomed. Chromatogr., 22: 830–834. doi: 10.1002/bmc.998
- Issue online: 18 JUL 2008
- Version of Record online: 2 APR 2008
- Manuscript Accepted: 12 DEC 2007
- Manuscript Received: 30 NOV 2007
- Department of Biotechnology, New Delhi
- Atropa acuminata;
- DART MS analysis;
- hairy root culture
Agrobacterium rhizogenes-mediated ‘hairy root’ cultures were established in Atropa acuminata. The chemical profiling of the hairy roots was carried out by a new mass spectrometric technique, direct analysis in real time (DART). The intact hairy roots were directly analyzed by holding them in the gap between the DART ion source and mass spectrometer. Two alkaloids, atropine and scopolamine, were characterized. The structural confirmation of the two alkaloids was made through their accurate molecular formula determinations. This is the first report of establishing hairy roots in A. acuminata as well as application of the DART technique for the chemical profiling of its hairy roots. Copyright © 2008 John Wiley & Sons, Ltd.