Abbreviations: MA = mycophenolic acid: DAC = 20,25-diazacholesterol.
Radiogas chromatography mass spectrometry in the selected ion monitoring mode†
Article first published online: 11 APR 2005
Copyright © 1980 John Wiley & Sons, Ltd.
Biological Mass Spectrometry
Volume 7, Issue 6, pages 259–264, June 1980
How to Cite
Doerfler, D. L., Rosenblum, E. R., Malloy, J. M., Naworal, J. D., McManus, I. R. and Campbell, I. M. (1980), Radiogas chromatography mass spectrometry in the selected ion monitoring mode. Biol. Mass Spectrom., 7: 259–264. doi: 10.1002/bms.1200070607
- Issue published online: 11 APR 2005
- Article first published online: 11 APR 2005
- Manuscript Received: 18 JAN 1980
The value of selected ion monitoring in analyzing biological radio isotope incorporation experiments by radiogas chromatography mass spectrometry is illustrated with reference to the biosynthesis of the mycotoxin mycophenolic acid and the mode of action of the anticholesterolemic drug 20,25-diazacholesterol. It is shown that the increased sensitivity and specificity of the selected ion monitoring mode detector permits straightforward detection and identification of the relatively small cellular pools associated with metabolic intermediates. The computer program RADSIM is described. Problems that still exist in using radiogas gas chromatography mass spectrometry technology to analyse isotope incorporation experiments are discussed.