Mass spectroscopic sequence analysis of oligonucleotides
Article first published online: 11 APR 2005
Copyright © 1985 John Wiley & Sons, Ltd.
Biological Mass Spectrometry
Volume 12, Issue 9, pages 514–524, September 1985
How to Cite
Grotjahn, L., Blöcker, H. and Frank, R. (1985), Mass spectroscopic sequence analysis of oligonucleotides. Biol. Mass Spectrom., 12: 514–524. doi: 10.1002/bms.1200120914
- Issue published online: 11 APR 2005
- Article first published online: 11 APR 2005
- Manuscript Accepted: 7 AUG 1984
- Manuscript Received: 18 MAY 1984
Negative ion fast atom bombardment (FAB) mass spectroscopy is used for sequencing fully deprotected oligonucleotides, 5′-tritylated oligonucleotides and negatively charged protected oligonucleotides. The mass spectrometric bidirectional sequence analysis allows characterization of modified oligonucleotides, even those where all classical sequencing methods have so far failed. Examples from each class of compound are illustrated and the problems and methodology are discussed.