Applied Cellular Physiology and Metabolic Engineering
Assessments of growth conditions on the production of cyanophycin by recombinant Escherichia coli strains expressing cyanophycin synthetase gene
Article first published online: 17 JAN 2012
Copyright © 2012 American Institute of Chemical Engineers (AIChE)
Volume 28, Issue 2, pages 358–363, March/April 2012
How to Cite
Tseng, W.-C., Fang, T.-Y., Cho, C.-Y., Chen, P.-S. and Tsai, C.-S. (2012), Assessments of growth conditions on the production of cyanophycin by recombinant Escherichia coli strains expressing cyanophycin synthetase gene. Biotechnol Progress, 28: 358–363. doi: 10.1002/btpr.1513
- Issue published online: 10 APR 2012
- Article first published online: 17 JAN 2012
- Accepted manuscript online: 27 DEC 2011 11:38AM EST
- Manuscript Revised: 8 DEC 2011
- Manuscript Received: 30 AUG 2011
- the National Science Council at Taiwan. Grant Number: NSC-97-2221-E-011-001-MY3
Additional Supporting Information may be found in the online version of this article.
|BTPR_1513_sm_SuppFig1.doc||128K||Figure S-1. Schematic illustration of cphA gene into an expression vector pET-21b by a modified restriction-free cloning method. The cphA gene of Synechocystis sp. PCC 6803 was cloned into pET-21b(+) vector by a modified restriction-free cloning method. After three stages of PCR, the parental pET-21b(+) vector was degraded by DpnI, and the remaining products were transformed to competent E. coli.|
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